Figure 3. Characterisation of the Lin⁻CD45⁻ population after isolation with magnetic columns.

(A) Debris (<1 µm) is excluded from the negative fraction. (B) Gate shows the exclusion of Lin⁺/CD41a⁺ cells as main contaminants and the gating of Lin⁻CD45⁻ population. (C, D, E) CD34⁺, CXCR4⁺ and Nestin⁺ are detected in the Lin⁻CD45⁻ fraction. (F) CD133⁺ is not detected in the Lin⁻CD45⁻fraction. (C, D, E, and F percentages represent the mean from 4 different samples). Events analysed: >100,000.