Figure 4. Heterogeneity of the Lin⁻CD45⁻ population.

(A) SSC and FSC back gate show CXCR4⁺, CD34⁺, and Nestin⁺ subpopulations compared to specific size beads of 6 µm and the Lin⁻CD45dimCD34⁺ (black); they have the same range of size in FSC but are allocated differently in SSC. (B) The box plot shows the percentage of CD34⁺, CXCR4⁺ and Nestin⁺ cells; note that Nestin⁺ cells are the larger population within the Lin⁻CD45⁻ cell fraction. (n = 4; *p<0.05/**p<0.005). (C) Gate shows that CXCR4⁺ cells are negative for CD34 (D) Gate shows Nestin⁺ CD34⁻ and Nestin⁺ CD34⁺ cells. (C and D percentages represent the mean from 4 different samples).