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. 2013 Jun 28;8(6):e67968. doi: 10.1371/journal.pone.0067968

Figure 5. Expression of transcripts typical of pluripotent cells and CD133 in the Lin−CD45− population.

Figure 5

(A–C) Expression of the embryonic stem cell markers, SSEA-4 and OCT3/4 but not of SOX2 are detected by flow cytometry (percentage represents the mean from 5 different samples). (D) SSC and FSC back gate shows SSEA-4 + and OCT3/4 + subpopulations compared to specific size beads of 6 µm and the Lin−CD45dimCD34+ (black); they are in the same size range both in FSC or SSC. (E) SOX2, OCT3/4 and NANOG transcripts are detected by RT-PCR. (F and G) Expression of Nestin and CD133 markers by qPCR in human neural (hNSC), in the Lin−CD45− fraction, and mesenchymal (MSC) stem cells. Nestin is expressed in both Lin−CD45− cells and MSCs cells though at a much lower level than in hNSC. Note that CD133 mRNA is not detected in the Lin−CD45− fraction.