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. 2013 Jun 28;8(6):e68308. doi: 10.1371/journal.pone.0068308

Figure 3. Memory, but not naïve B cells are significantly activated in chronically infected HCV patients.

Figure 3

PBMCs were freshly isolated and analyzed by flow cytometry as in Figure 2. Antibodies to CD183, CD71, CD86, CD69, HLA-DR and CD40 were added in combinations with CD19 and CD27 to gate on memory (CD19+CD27+) and naïve (CD19+CD27−) B cells. The percent positive was calculated based on mouse isotype controls and the geometric mean fluorescent intensities (MFI) for each marker were determined. HLA-DR and CD40 are expressed by all B cells therefore only the MFI is shown. Horizontal lines on graphs represent median values. For CD71 analysis, only 48 HCV and 47 healthy control samples were stained. ***, P<0.001, **, P<0.01, *, P<0.05, n.s., not significant.