Figure 1. The proteolytic activity of Natterins is sufficient for the generation of long-lasting Th2 polarized humoral response.

BALB/c mice were immunized with active or inactivated Natterins on day 0 and boosted 7, 21 and 28 days later. Serum anti-Natterins titers were determined by ELISA (A, C, and E). The anaphylactic IgG1 and IgE Abs were examined by PCA (B and D). Each group consisted of at least five male mice, and representative data from three repeated experiments are shown. *p<0.05 compared to control-mice, and #p<0.05 compared to active Natterins. The dotted line represents the Abs level of control non-immunized mice before immunization with adjuvant at day 0.