Figure 3. Natterins induce the B1b cell response.

A representative dot plot of B1b cells (B220^posCD5^neg from CD3-negative gated cells) obtained at 48, 74 and 120 d after immunization with active or inactivated Natterins is shown in peritoneum (A, D), spleen (B, E) and BM (C, F). The bars representative of the absolute numbers of B220^posCD5^neg cells were determined from total mononuclear cells by multiparametric flow cytometer using using Rat IgG2bk FITC-anti-mouse CD3, Rat IgG2ak PE-anti-mouse CD5, and Rat IgG2ak PerCP-Cy5-anti-mouse CD45R/B220. *p<0.05 compared to control-mice, and # p<0.05 compared to active Natterins.