Figure 4. The chronic maintenance of B2 cells in spleen and in the bone marrow is dependent on proteolytic activity of Natterins.

A representative dot plot of B2 cells (B220^posCD23^pos from CD3-negative gated cells) obtained at 48, 74 and 120 d after immunization with active or inactivated Natterins is shown in peritoneum (A, D), spleen (B, E) and BM (C, F). The bars representative of the absolute numbers of B220^posCD23^pos cells were determined from total mononuclear cells by multiparametric flow cytometer using Rat IgG2bk FITC-anti-mouse CD3, Rat IgG2ak PE-anti-mouse CD23, Rat IgG2ak PerCP-Cy5-anti-mouse CD45R/B220. *p<0.05 compared to control-mice, and # p<0.05 compared to active Natterins.