Figure 6. Determination of the bioenergetic flexibility of MB231 and MB231^shHIF-1α cells during de-oxygenation and re-oxygenation MB231 (circles) and MB231 ^shHIF-1α (square) cells were equilibrated to atmospheric OT and is de-oxygenated to 4% and then to 1%.

The chamber is re-oxygenated step-wise to 4% and then back to atmospheric OT. Several OCR measurements were made at each step and were plotted over time. A representative O₂ trace (dotted line) during the course of the experiment is shown for reference.