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. 2013 Jun 28;8(6):e67766. doi: 10.1371/journal.pone.0067766

Figure 1. Overexpression of CD9 in HT1080 cells does not alter the expression of other tetraspanins.

Figure 1

(A) The cell surface expression of CD9 on wild-type HT1080 cells was assessed using flow cytometric analysis after binding a monoclonal antibody specific for CD9 (mAb7, shaded histogram). A non-specific isotype-matched antibody (IgG) was used as a negative control (unshaded histogram). (B, C) Total RNA was collected from Mock- and CD9-HT1080 cells, reverse transcribed to cDNA, and probed using primers specific for integral tetraspanins using qRT-PCR. The fold change in mRNA expression of tetraspanins was calculated from resulting cycle threshold values normalized to cyclophilin D. (D) Flow cytometry was used to assess the cell surface expression of key tetraspanins on the cell surface of Mock- (left panels) and CD9- (right panels) HT1080 cells **, p<0.001.