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. 2013 Jun 28;8(6):e67348. doi: 10.1371/journal.pone.0067348

Figure 1. Expression profiles of calcium transporters in lactation.

Figure 1

Mouse mammary tissue, starting from day 10 prior to parturition, was evaluated for the expression of (A) SPCA1, and (B) SPCA2 by Western blotting. Loading was normalized relative to tissue DNA concentrations, and expressed relative to starting levels at Day -10. C) RT-PCR of mRNA for isoforms of SPCA, Orai, PMCA and STIM proteins at distinct time points of mammary development, including pre-pregnancy (virgin), parturition (Day 0), lactation (Day 5) and involution (Day 5 after removal of pups). Transcripts are grouped as early, mid and unchanged, according to their time of induction following lactation on Day 0. D) SPCA2 was immunoprecipitated from lactating mouse mammary tissue using polyclonal rabbit anti-hSPCA2 peptide antibody or rabbit IgG as control. Orai1 was detected as a co-immunoprecipitate by immunoblotting (IB).