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. 2013 Jun 28;8(6):e67303. doi: 10.1371/journal.pone.0067303

Figure 7. The effects of PRP or HGF treatment on COX-1 protein expression in tendon cell culture by immunostaining.

Figure 7

Tendon cells in culture were treated with IL-1β, PRP, HGF, and/or HGF antibody (AB) to determine COX-1 expression using goat anti-COX-1 antibody and Cy-3 conjugated donkey anti-goat IgG antibody. Red represents COX-1 protein; and blue represents nuclei stained with Hoechst 33342. IL-1β treatment induced a high level of COX-1 expression in tendon cells compared to the control conditions (A); however, such an increase in COX-1 was markedly reduced by PRP or HGF treatments. The addition of HGF antibody to tendon cell cultures decreased the reduction by PRP or HGF treatments. A: SF (control conditions); B: IL-1β (1 ng/ml); C: 2%PRP+ IL-1β; D: 10%PRP+IL-1β; E: HGF+IL-1β; F: 2%PRP+IL-1β+AB; G: 10%PRP+IL-1β+AB; H: HGF+IL-1β+AB; I: 2%PRP; J: 10%PRP; K: HGF (1 ng/ml) and L: HGF+AB (10 ng/ml). Bar: 100 µm.