Figure 8. The effects of PRP or HGF treatment on COX-2 protein expression in tendon cell culture by immunostaining.

Tendon cells in culture were treated with IL-1β, PRP, HGF, and/or HGF antibody (AB) to determine COX-2 expression using goat anti-COX-2 antibody and Cy-3 conjugated donkey anti-goat IgG antibody. Red represents COX-2 protein; and blue represents nuclei stained with Hoechst 33342. Similar to COX-1 results ( Figure 7 ), PRP or HGF treatments markedly reduced COX-2 expression, and the combined use of HGF antibody with PRP or HGF decreased the reduction effects produced by either PRP or HGF treatment alone. Label abbreviations (A to L) are the same as in Figure 7 . Bar: 100 µm.