Figure 10. The effects of PRP, PPP, HGF and HGF antibody on PGE₂ production in wounded mouse Achilles tendons in vivo.

A. PRP and PPP injections. Injections with PRP significantly reduced PGE₂ levels in tendons on days 1, 3, and 5 post-treatment. However, on day 12, PRP’s effect on PGE₂ production in tendon tissues was little, if any. On day 0, little difference was observed in PGE₂ levels between PRP-treated and non-treated tendons. However, PPP treatment did not significantly decrease PGE₂ levels in the tendon tissues at all time points post-treatment (P>0.05). However, a small suppression effect on days 1, 3, and 5 likely occurred, as PPP-treated tendons had consistently lower PGE₂ levels than control tendons injected with saline. B. HGF injection. Injection with HGF decreased PGE₂ production in wounded Achilles tendons, producing results similar to PRP injections except on day 12, when HGF injection still significantly decreased PGE₂ production (*P<0.01 is with respect to control tendons given saline injections). Addition of HGF+HGF antibody (AB) largely negated the suppressive effect of HGF. C. PGE₂ production in mouse tendons injected with PRP and HGF antibody. Injection of PRP along with HGF antibody into wounded Achilles tendons restored PGE₂ levels to normal on all days 1, 3, 5 and 12. The data in Figure 10A, B, C are expressed as mean ± SD, and n  = 4 (4 mice).