Table 3. Average game centrality (GC) values for E. coli methionyl-tRNA synthetase amino acids.

	Average Game Centrality (GC) of substrate-free proteina	Average Game Centrality (GC) of substrate-bound protein	Game Centrality (GC) decrease
Catalytic domainb	0.69	0.60	0.09
• Rossmann-fold-1(active centre)	0.73	0.56	0.17
• Rossmann-fold-2	0.68	0.62	0.06
• Stem contact fold (KSMKS)	0.65	0.61	0.04
Connecting peptide (CP) domain	0.50	0.36	0.14
tRNA anticodon-binding domain	0.63	0.41	0.22
Signaling amino acids [39]	0.79	0.73	0.06
Complete Met-tRNA-synthetase	0.62	0.47	0.15

^aProtein structure network of E coli methionyl-tRNA-synthetase was constructed, Prisoner’s dilemma game was simulated, and game centrality measures were calculated as described in Methods.

^bDomains from top to bottom: the catalytic domain including the Rossmann-fold-1 (catalytic function), Rossmann-fold-2 and stem contact fold (KMSKS) sub-domains; the connecting peptide (CP) domain; the anticodon binding, carboxy-terminal domain, 43 signaling amino acids involved in the transmission of conformational change as shown by Ghosh and Vishveshwara [46], whole methionyl-tRNA synthetase.