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Expression SCARB2 in RD and HT29 cells. Total intracellular RNA were harvested from RD and HT29 cells, converted to cDNA and measured by quantitative real time polymerase chain reaction (qRT-PCR) with primers specific to SCARB2 and ACT. Primers for SCARB2 and ACT were designed to span exon-exon boundaries to give a single PCR product of 89 bp and 198 bp respectively. The presence of SCARB2 in HT29 further supports HT29 cells as a viable in vitro model to study EV71 pathogenesis.
