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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Structure. 2013 May 23;21(6):900–909. doi: 10.1016/j.str.2013.04.016

Figure 4. ‘Gold standard’ FSC plot for the EMAN2 reconstruction.

Figure 4

The EMAN2 refinement of the full data set produced a ‘gold standard’ resolution of about 17 Å. Splitting the data into more homogeneous subsets resulted in an additional map (not shown) with a ‘gold standard’ resolution of 14.7 Å. This demonstrates that resolution is limited by structural variability for this data set. If the data quality were limiting the resolution, splitting the data would not improve the resolution.