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. 2013 Jun 12;169(5):1058–1071. doi: 10.1111/bph.12185

Figure 1.

Figure 1

STS attenuates OGD-induced H9c2 cardiomyocyte apoptosis. Cells were subjected to 6 h OGD and 18 h recovery with or without STS treatment. Apoptosis of cells was determined with (A) Hoechst 33342-based fluorescence microscopy. Arrowheads in the pictures indicate the nuclei of apoptotic cells, (B) Annexin V-FITC-based flow cytometry (Annexin V-FITC +/PI- population of gated cells was measured with CellQuest software and presented as an apoptosis ratio). (C) Caspase-3 and caspase-8 activities and (D) MMP were also determined, after OGD/R and treatment with STS. Values are presented as means ± SD from three independent experiments. ###P < 0.001, significantly different from control, *P < 0.05, **P < 0.01, **P < 0.001, significantly different from OGD/R alone. (E) Cell viability was determined using the MTT assay. STS (10 μM) induced no cell toxicity (P > 0.05).