Figure 3.

Effect of UCB1244283 on the binding characteristics of [³H]UCB30889. Saturation binding curves of [³H]UCB30889 (A) on human recombinant SV2A expressed in HEK cells and (B) on rat cortex. Membranes were incubated with increasing concentrations of [³H]UCB30889 for 60 min at 37°C with (open symbols) or without (filled symbols) 10 μM of UCB1244283. Non-specific binding (NSB) was determined as the residual binding observed in the presence of 1 mM levetiracetam. The insets are the Scatschard plots from the transformed data. Specific binding = total binding – NSB. Results are representative of three independent experiments.