Table 2.
Effect of UCB1244283 on the Kd and Bmax values of [3H]UCB30889 on rat cortex and human recombinant SV2A expressed in HEK cells at 4°C and 37°C
| Rat cortex | Human recombinant SV2A | |||
|---|---|---|---|---|
| + UCB1244283 | + UCB1244283 | |||
| Kd (nM) | ||||
| 4°C | 88 ± 8 | 37 ± 9** | 64 ± 5 | 13 ± 5*** |
| 37°C | 247 ± 10 | 83 ± 11*** | 153 ± 23 | 33 ± 8*** |
| Bmax (pmol·mg−1) | ||||
| 4°C | 14.6 ± 3.8 | 22.0 ± 7.3 | 66.6 ± 17.1 | 118.2 ± 20.4* |
| 37°C | 14.9 ± 1.7 | 25.1 ± 0.9*** | 69.1 ± 13.8 | 130.2 ± 14.5** |
Data are represented as mean ± SD (n = 3, *P < 0.05; **P < 0.01; ***P < 0.001, unpaired Student's t-test between condition with and without UCB1244283).