Table 1.
Features of standard isotopic labeling using U-13C versus Isotopic Ratio Outlier Analysis™.
| Feature | U-13C (99 atom %) | IROA |
|---|---|---|
| Sample throughput | High to medium | High to medium |
| Removal of sample-to-sample variance | Yes – control and experimental samples are prepped and analyzed together, removing variability | Yes – control and experimental samples are prepped and analyzed together, removing variability |
| Removal of ion suppression | Yes – ionization is similar for isotopomers | Yes – ionization is similar for isotopomers |
| Reduction of the number of samples to be analyzed | Yes – control and experimental samples pooled and run as a single sample, reducing the number of samples to be analyzed by half | Yes – control and experimental samples pooled and run as a single sample, reducing the number of samples to be analyzed by half |
| Distinguishes artifacts and noise | No | Yes – allows classification of all peaks as compounds or artifacts |
| Metabolite coverage | Targeted | Global |
| Metabolite Identification | No | Number of carbon atoms and residual mass defined |
IROA: Isotopic Ratio Outlier Analysis™.