Table 1.
Feature | U-13C (99 atom %) | IROA |
---|---|---|
Sample throughput | High to medium | High to medium |
Removal of sample-to-sample variance | Yes – control and experimental samples are prepped and analyzed together, removing variability | Yes – control and experimental samples are prepped and analyzed together, removing variability |
Removal of ion suppression | Yes – ionization is similar for isotopomers | Yes – ionization is similar for isotopomers |
Reduction of the number of samples to be analyzed | Yes – control and experimental samples pooled and run as a single sample, reducing the number of samples to be analyzed by half | Yes – control and experimental samples pooled and run as a single sample, reducing the number of samples to be analyzed by half |
Distinguishes artifacts and noise | No | Yes – allows classification of all peaks as compounds or artifacts |
Metabolite coverage | Targeted | Global |
Metabolite Identification | No | Number of carbon atoms and residual mass defined |
IROA: Isotopic Ratio Outlier Analysis™.