Fig. 3.

A.) BSA-anchored biotin-streptavidin-biotin sandwich layer is used to immobilize a DNA capture probe on a glass coverslip. The patterned glass is sealed in a chamber using either oxygen plasma or our polymer-graft and baked for 4 hours. The Cy5-modified target oligo-DNA is introduced and after hybridization for 2 hours and rinsing for 30 minutes, the device is imaged using Typhoon scanner. B.) While the device sealed with oxygen plasma was almost devoid of any patterns (top), the device bonded using our bonding technique clearly shows the patterned capture probe (bottom), thereby demonstrating our method’s compatibility with biological molecules (fluorescent squares are 200-by-200 microns).