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. 2001 Apr 15;107(8):985–993. doi: 10.1172/JCI10734

Figure 3.

Figure 3

Reactivity of human anti-R3 Ab’s. Western blot analysis with affinity-purified anti-R3 Ab’s from human chagasic sera. (a) Reactivity against sCha (20 kDa) antigen in Jurkat cells and a 36-kDa antigen present in T. cruzi amastigotes is shown. (b) sCha is expressed in mouse heart and the mouse macrophage J774 cell line. (c) Competitive R3 peptide ELISAs show that R3 homologous peptides from human (dark gray boxes), mice (light gray boxes), and T. cruzi (open boxes) competed out the binding of either affinity-purified anti-R3 Ab’s or whole sera (Ch+HS) from human chagasic patients to the human R3 peptide (filled boxes).