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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Cell Biol Int. 2012 Oct 1;36(10):901–907. doi: 10.1042/CBI20110586

Fig. 2. The proximity Ligation Assay produces red fluorescent spots if two proteins are in close association.

Fig. 2

Ishikawa cells were sparsely grown on 16-well Lab-Tek chamber slide for 48 h and then subjected to proximate ligation assay. A) PLA assay without primary antibodies B) PLA assay with primary antibodies against DAF and caveolin1 C) PLA assay with primary antibodies against DAF and ganglioside GM1.