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. Author manuscript; available in PMC: 2014 Apr 26.
Published in final edited form as: J Nat Prod. 2013 Mar 21;76(4):685–693. doi: 10.1021/np300913h

Figure 4.

Figure 4

Thailanstatins (1–3) inhibit pre-mRNA splicing in vitro. FR901464 (4) and 1–3 were added to splicing reactions containing 32P-labeled CDC14–15 pre-mRNA at the final concentrations indicated. AMP-PNP (5 mM) and DMSO were used as positive and negative controls, respectively. RNA was purified and resolved on denaturing PAGE and gels were autoradiographed at −80 °C. Identities of splicing intermediates are depicted to the right of gels.