Table 1.
Comparison of genes and deduced proteins between the thailanstatin biosynthetic gene cluster (tst) from Burkholderia thailandensis MSMB43 (GenBank accession no. JX307851) and the FR901464 biosynthetic gene cluster (fr9) from Pseudomonas sp. No. 2663 (GenBank accession no. HM047288).
| The tst gene cluster | The fr9 gene cluster | DNA sequence identity / gap |
Protein sequence similarity / identity |
Confirmed or deduced protein function |
||
|---|---|---|---|---|---|---|
| Gene | Deduced protein (# of aa) |
Gene | Deduced protein (# of aa) |
|||
| tstA | TstA (276) | fr9A | FR9A (294) | 71% / 1% | 71% / 57% | LuxR-type transcription regulator |
| tstC | TstC (4,997) | fr9C | FR9C (5,061) | 78% / 2% | 81% / 75% | PKS / Hybrid PKS-NRPS |
| tstDEF | TstDEF (8,036) | fr9D | FR9D (1,170) | 81% / 3% | 85% / 79% | Hybrid PKS-NRPS |
| fr9E | FR9E (1,188) | 79% / 3% | 81% / 76% | PKS | ||
| fr9F | FR9F (4,904) | 81% / 2% | 81% / 75% | PKS | ||
| tstGH | TstGH (4,855) | fr9G | FR9G (3,017) | 66% / 3% | 80% / 74% | PKS |
| fr9H | FR9H (2,662) | 69% / 4% | 83% / 77% | PKS | ||
| tstI | TstI (3,061) | fr9I | FR9I (3,052) | 67% / 3% | 75% / 69% | PKS |
| tstJ | TstJ (294) | fr9J | FR9J (296) | 81% / 1% | 86% / 80% | Acyltransferase |
| tstK | TstK (492) | fr9K | FR9K (429) | 86% / <1% | 88% / 82% | HMG-CoA synthase |
| tstL | TstL (261) | fr9L | FR9L (251) | 80% / <1% | 88% / 80% | Enoyl-CoA hydratase |
| tstM | TstM (79) | fr9M | FR9M (79) | 78% / <1% | 92% / 78% | Acyl carrier protein |
| tstN | TstN (422) | fr9N | FR9N (421) | 79% / <1% | 85% / 79% | KetosynthetaseS |
| tstO | TstO (392) | fr9O | FR9O (377) | 75% / 4% | 92% / 74% | Acyltransferase |
| tstB | TstB (322) | fr9B | FR9B (360) | NSHa | 53% / 35% | Acyltransferase |
| tstR | TstR (482) | fr9R | FR9R (482) | 85% / <1% | 93% / 87% | Cytochrome P450 |
| tstQ | TstQ (145) | fr9Q | FR9Q (145) | 73% / <1% | 88% / 69% | Cyclase |
| tstP | TstP (341) | fr9P | FR9P (341) | 81% / <1% | 91% / 85% | Dioxygenase |
| -- | -- | fr9S | FR9S (152) | --b / -- | -- / -- | Acetyltransferase |
| -- | -- | fr9T | FR9T (467) | -- / -- | -- / -- | Diguanylate cyclase |
NSHa, no significant homology; --b, not available.