Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Nat Methods. 2013 May 12;10(7):671–675. doi: 10.1038/nmeth.2479

Figure 1. BEST method.

Figure 1

(a) A pool of barcoded plasmids is transformed into the diploid strain from a cross. (b) Transformants are then grown on selection and ~10,000 transformed colonies are pooled and sporulated. During meiosis each spore of a tetrad inherits a copy of the barcoded plasmid. (c) Tetrads are separated from unsporulated cells by FACS and collected on agar plates, where they are digested and disrupted to allow each spore to form a colony. (e) Colonies are then picked into 96 well plates, phenotyped and genotyped. During genotyping the plasmid barcode is read and used to identify the four members of each tetrad.