Table 3.
[diC8-PI(4,5)P2] | 1 μM | 3 μM | 10 μM | 30 μM | 100 μM | 300 μM |
---|---|---|---|---|---|---|
Kv7.2 WT (n = 10) | 0.088 ± 0.032 | 0.108 ± 0.048 | 0.163 ± 0.056 | 0.292 ± 0.094 | 0.452 ± 0.087 | 0.727 ± 0.072 |
K327A (n = 10) | 0.071 ± 0.045 | 0.113 ± 0.056 | 0.162 ± 0.058 | 0.256 ± 0.059 | 0.501 ± 0.072 | 0.686 ± 0.067 |
R325A (n = 8) | 0.020 ± 0.007 | 0.027 ± 0.009 | 0.040 ± 0.015 | 0.104 ± 0.060 | 0.171 ± 0.083* | 0.294 ± 0.110** |
R325A/K327A (n = 8) | 0.013 ± 0.006 | 0.016 ± 0.006 | 0.0024 ± 0.011* | 0.037 ± 0.013* | 0.055 ± 0.013** | 0.180 ± 0.067*** |
*P < 0.04, **P < 0.004, ***P < 0.001, significantly different from the wild type Kv7.2