Table 3.

Effect of increasing concentrations of diC₈-PI(4,5)P₂ on P _open of wild-type and mutated Kv7.2 channels recorded in excised inside-out membrane patches: mean data

[diC8-PI(4,5)P2]	1 μM	3 μM	10 μM	30 μM	100 μM	300 μM
Kv7.2 WT (n = 10)	0.088 ± 0.032	0.108 ± 0.048	0.163 ± 0.056	0.292 ± 0.094	0.452 ± 0.087	0.727 ± 0.072
K327A (n = 10)	0.071 ± 0.045	0.113 ± 0.056	0.162 ± 0.058	0.256 ± 0.059	0.501 ± 0.072	0.686 ± 0.067
R325A (n = 8)	0.020 ± 0.007	0.027 ± 0.009	0.040 ± 0.015	0.104 ± 0.060	0.171 ± 0.083*	0.294 ± 0.110**
R325A/K327A (n = 8)	0.013 ± 0.006	0.016 ± 0.006	0.0024 ± 0.011*	0.037 ± 0.013*	0.055 ± 0.013**	0.180 ± 0.067***

*P < 0.04, **P < 0.004, ***P < 0.001, significantly different from the wild type Kv7.2