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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biomed Chromatogr. 2012 Apr 20;27(1):58–66. doi: 10.1002/bmc.2748

Table 1.

Gradient mobile phase timing, flow rate and composition for the reversed-phase chromatographic separation of γ-tocotrienol.

Time (min) Flow Rate (µL/min) Solvent A* (%) Solvent B* (%)
0 1000 70 30
1 1000 70 30
2 1000 0 100
8.5 1000 0 100
9 1000 70 30
9.5 1000 70 30
*

Solvent A was water, and solvent B was 0.2% formic acid in acetonitrile.