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. Author manuscript; available in PMC: 2013 Nov 7.
Published in final edited form as: Cell Metab. 2012 Oct 18;16(5):588–600. doi: 10.1016/j.cmet.2012.09.014

Fig. 2. GDS favors lipolysis through Htr2b receptor expressed in adipocytes.

Fig. 2

(A) Glycerol and (B) FF As release from mouse epigonadal fat explants stimulated with indicated doses of serotonin (n > 5). (C) Relative expression of indicated serotonin receptors in adipose tissue of fed and fasted mice. Plasma levels of (D) glycerol, (E) FF As and (F) β-hydroxybutyrate in mice lacking Htr2b in adipose tissue (deletion by aP2-Cre – indicated on graphs Htr2bfatΔ/Δ (aP2)) fasted for indicated times (n ≥ 20). Plasma levels of (G) glycerol and (H) FFAs in mice lacking Htr2b in adipose tissue (deletion by Adiponectin-Cre – indicated on graphs Htr2bfatΔ/Δ (Aq) fasted for indicated times (n > 6). (I) Epigonadal fat pad weight to body weight ratio in fed and fasted Htr2bf/f and Htr2bfatΔ/Δ(aP2) mice (n > 12). (J) Glycerol and (K) FFAs levels in Htr2bf/f and Htr2bfatΔ/Δ(aP2) mice implanted with serotonin-releasing pellets. (L) Glycerol and (M) FFAs release from epigonadal fat explants isolated from Htr2bf/f and Htr2bfatA/A(aP2) mice and stimulated with 50μM serotonin or 1μM isoproterenol (n = 5). (N) Western blot analysis of expression and activation of indicated proteins in fat explants isolated from Htr2bf/f and Htr2bfatΔ/Δ(aP2) mice and stimulated with 50μM serotonin. (O) Western blot analysis of expression and activation of indicated proteins in fat pads isolated from Htr2bf/f and Htr2bfatΔ/Δ(aP2) mice fasted for 48h. Data represented as mean +/− SEM, *P < 0.05, ***P < 0.001.