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. Author manuscript; available in PMC: 2013 Jun 30.
Published in final edited form as: J Neurophysiol. 2006 Apr;95(4):2664–2677. doi: 10.1152/jn.00752.2005

FIG. 2.

FIG. 2

Experimental demonstration of postinhibitory facilitation. A: patch-clamp recordings (in vitro gerbil medial superior olive [MSO], P22) of membrane potential for a pair of inhibitory and subthreshold excitatory inputs, Gex = 20 nS, are applied with the dynamic-clamp method with varying preceding times of inhibition, Ginh = 100 nS, (see text for experimental details). B: elimination of PIF after application of the IKLT blocker dendrotoxin I (DTXI). Over the same range of δ as in A the inhibitory conductance transient (IPSG) does not facilitate the subthreshold excitatory conductance (EPSG) to generate a spike. C: Gex was increased by 5% relative to B for just-suprathreshold EPSG, which was preceded by an IPSG in the presence of DTXI. In all cases of the δ-range expected to show facilitation the IPSG suppressed spiking–no facilitation. To compensate for membrane conductance changes in B and C, the amplitudes of Gex and Ginh were reduced approximately 2-fold from the control conditions.