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. 2013 Jun 20;3(3):302–311.

Figure 1.

Figure 1

TRβ inhibits the proliferation of MCF-7 cells. A: TRβ was stably expressed in MCF-7 cell (lanes 3 and 4), but not in control MCF-7-Neo cells (lanes 1 and 2). The Western blot analysis was carried out as described in Materials and Methods. Two representative results are shown using two independent clones: MCF-7-Neo#1 (lane 1) and MCF-7-Neo#12 (lane 2) and MCF-7-TRβ#18 (lane 3) and MCF-7-TRβ#19 (lane 19). B: E2-dependent cell proliferation of MCF7 was inhibited by the expression of TRβ in the presence of T3. Cells with or without hormone (E2 and T3) treatment are as marked. Cell growth was analyzed as described in Materials and Methods. Data are expressed as mean ± standard error (SE) (n=3).