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. 2013 Jun 10;123(7):2832–2849. doi: 10.1172/JCI65859

Figure 3. Identification of TLR4/NANOG–dependent oncogenes, Igf2bp3 and Yap1, from functional screening of a lentiviral cDNA library.

Figure 3

(A) Strategy of in vitro identification of liver oncogenes, with oval cells infected with the TIC library but not with the control cell library forming colonies. (B) Increased expression of Igf2bp3 and Yap1 in the TICs as confirmed by qPCR is further upregulated by LPS treatment. Nanog shRNA significantly reduces these inductions. (C) Schematic diagrams of Igf2bp3 and Yap1 promoters depicting the locations of NANOG consensus binding sequences (orange boxes) and the areas analyzed by ChIP (1–16 black boxes for Igf2bp3 and 1–9 black boxes for Yap1). NANOG ChIP-qPCR data for TICs from the Ns5a Tg model are shown below, with NANOG enrichment in the regions 11 and 12 within NANOG 1 and 2 sites for the Igf2bp3 promoter and in the region 5 encompassing the NANOG 1 site of the Yap1 promoter.