Figure 6.

Combined pharmacological PI3K and Hsp90 inhibition leads to enhanced apoptotic effects in MM cells. (A and B) Viability analyses by annexin V-FITC/PI staining upon treatment of INA-6 or MM.1S cells with fixed concentrations of either PI103, AUY922 or a combination of both drugs for 3 days. Three independent experiments were conducted for each MM cell line. (C and D) Combination analyses (three independent experiments) for PI103 and AUY922 with combination index (CI) determination according to the method of Chou (fixed ratio design, simultaneous drug addition, 3 days of drug treatment). (E) Primary MM samples obtained from the peripheral blood of a patient with plasma cell leukemia or isolated from bone marrow aspirates from 24 MM patients were co-cultured with primary BMSC and treated as indicated for 72 h prior to staining with annexin V-FITC/PI. (*) indicates statistically significant differences (P<0.05).