FIGURE 11.

GDH clips free as well as chromatin-bound histone H3. A, shown is a protease assay with the standard preparation of brain core histones and pure recombinant histones H3, H2B, H2A, and H4. B, integrity of soluble chromatin was evaluated after incubation for 7 h at 4 °C or at 37 °C without and with GDH (left panel), DNA purification, and electrophoresis on 1.2% agarose gel (right panel). M stands for DNA marker/ladder. C, shown is a protease assay with soluble chromatin incubated for 7 h at 37 °C. D, shown is a comparison of protease activity of GDH on free core histones and chromatin-bound histones using time point assay (upper panel) and its quantification (lower panel) after incubations for 1, 2, 4, or 10 h at 37 °C.