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Purification profile of GDH. A, shown is purification of GDH (Fig. 1B). The active fractions after each step of purification were loaded on 10% SDS-polyacrylamide gel (upper panel) to analyze the purification profile of GDH. These fractions were also probed by immunoblotting using anti-GDH (anti-GLUD1) antibody to confirm the purification of GDH (lower panel). B, a purification table of GDH is shown.
