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. 2013 May 14;288(26):18743–18757. doi: 10.1074/jbc.M113.462531

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Immunoprecipitation of GDH. A, immunoprecipitation (IP) fractions eluted from anti-GDH antibody beads or unmodified beads as control were resolved and visualized on 10% silver-stained SDS-PAGE (upper panel). Western blot analysis of the fractions for the detection of GDH was conducted using anti-GDH (anti-GLUD1) antibody (lower panel). B, shown is a H3 protease activity assay with immunoprecipitation fractions and quantification of the activity. In, input. C, shown is thr presence of protease activity in the ER. A small volume from the fractions containing ER proteins was incubated with brain core histones that were then resolved by 15% SDS-PAGE. Un, undigested core histones. D, shown is an immunoblot analysis of microsomal and ER extract using anti-GDH antibody.