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Detection of polysialylated NCAM and ST8SiaII using human sperm. For Western blotting (WB) against NCAM (A) and ST8SiaII (B), polysialylated proteins were purified from mobile human sperm lysates using magnetic beads, which were coated with inactive endoN. Western blot analyses were performed with or without previous endoN treatment. Apparent molecular masses of standard proteins are indicated in kDa.
