FIGURE 2.

Knockdown of Olig1 selectively impairs cell motility induced by TGF-β. Cells were transfected with control or Olig1 siRNA. After 16 h, cells were used in the indicated assays. A, effects of Olig1 knockdown on TGF-β-induced cytostasis in NMuMG cells. Cells were treated with TGF-β (1 ng/ml) for 48 h and counted. siControl denotes a negative control oligonucleotide. Error bars represent S.D. B, effects of Olig1 knockdown on TGF-β-induced EMT. Cells were stimulated with TGF-β (1 ng/ml) for 24 h after knockdown of Olig1. Protein expression of a mesenchymal marker, N-cadherin (top panel) and an epithelial marker, E-cadherin (middle panel), are depicted. The bottom panel shows expression level of tubulin protein, as a loading control. C and E, chamber migration assay. Cells were stimulated with TGF-β (1 ng/ml, C) or BMP-4 (10 ng/ml, E). D and F, wound healing assay. Cells were stimulated with TGF-β (1 ng/ml, D) or BMP-4 (10 ng/ml, F). Quantitations are shown in the right. p values were determined by Student's t test. *, p < 0.01. G, effect of Smad2/3 knockdown on TGF-β-induced cell motility in chamber migration assay. Cells were transfected with control or Smad2/3 siRNA. After 48 h, cells were used in chamber migration assay.