TABLE 2.

Effect of LXCXE and E2F peptide ligands on RbAB thermodynamic stability

	ΔGUNF,LIGa	C50a	KD,BINDa	KD,BINDb
	kcal/mol	m	m	m
RbAB + E2F-TD	7.0 ± 0.2	3.9 ± 0.6	(8.6 ± 1.3) × 10−7	(1.9 ± 0.4) × 10−7 (26)c
RbAB + E7(16–31)	9.5 ± 0.1	5.3 ± 0.9	(1.1 ± 0.1) × 10−8	2.1 × 10−9 (56)d

^a Values determined in this study. Global fitting was performed on unfolding curves measured in urea at 1 μm RbAB concentration and 10 μm peptide using the m-value and ΔG_UNF value for the isolated RbAB domain determined in the urea unfolding of the isolated RbAB domain (Table 1). The measured CD and CSM signals were normalized prior to fitting.

^b Values obtained from literature.

^c K_D measured by isothermal titration calorimetry.

^d K_D measured by fluorescence anisotropy.