TABLE 3.

Effect of LXCXE and E2F peptide ligands on RbAB thermal stability

All measurements were performed at 2 μm RbAB and 20 μm peptides. Buffer was 20 mm sodium phosphate, pH 7, 2 mm DTT, and 0.01% Tween 20 at the specified NaCl concentration. Scan speed, 3 °C/min. NA, not assessed; pS, phosphoserine.

Peptide	Sequencea	ΔTm(app)		ΔGBINDb
		0.05 m NaCl	0.6 m NaCl	0.05 m NaCl	0.6 m NaCl
		°C		kcal/mol
E7(16–31)	QPETTDLYCYEQLNDS	10.6 ± 0.2	8.7 ± 0.2	−12.6	−10.6
E7(16–40)	QPETTDLYCYEQLNDSSEEEDEIDG	12.2 ± 0.2	8.8 ± 0.2	−13.1	−10.4
E7(16–40)PP	QPETTDLYCYEQLNDpSpSEEEDEIDG	12.5 ± 0.2	10.1 ± 0.2	−14.4	−11.0
E7N HPV-16	MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDG	12.5 ± 0.2	NA	−13.4	NA
E7N HPV-18	MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDG	12.2 ± 0.2	NA	−12.1	NA
E7N HPV-11	MHGRLVTLKDIVLDLQPPDPVGLHCYEQLEDSSEDEVDKVDK	7.9 ± 0.2	NA	−10.2	NA
E2F2-TD	DDYLWGLEAGEGISDLFD	9.4 ± 0.2c	NA	NA	NA

^a The HPV E7 LXCXE and the E2F-TD helix motifs are underlined and in bold.

^b Values were taken from Ref. 56.

^c Measurements were performed at 0.2 m NaCl.