TABLE 3.
Peptide | Sequencea | ΔTm(app) |
ΔGBINDb |
||
---|---|---|---|---|---|
0.05 m NaCl | 0.6 m NaCl | 0.05 m NaCl | 0.6 m NaCl | ||
°C | kcal/mol | ||||
E7(16–31) | QPETTDLYCYEQLNDS | 10.6 ± 0.2 | 8.7 ± 0.2 | −12.6 | −10.6 |
E7(16–40) | QPETTDLYCYEQLNDSSEEEDEIDG | 12.2 ± 0.2 | 8.8 ± 0.2 | −13.1 | −10.4 |
E7(16–40)PP | QPETTDLYCYEQLNDpSpSEEEDEIDG | 12.5 ± 0.2 | 10.1 ± 0.2 | −14.4 | −11.0 |
E7N HPV-16 | MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDG | 12.5 ± 0.2 | NA | −13.4 | NA |
E7N HPV-18 | MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDG | 12.2 ± 0.2 | NA | −12.1 | NA |
E7N HPV-11 | MHGRLVTLKDIVLDLQPPDPVGLHCYEQLEDSSEDEVDKVDK | 7.9 ± 0.2 | NA | −10.2 | NA |
E2F2-TD | DDYLWGLEAGEGISDLFD | 9.4 ± 0.2c | NA | NA | NA |
a The HPV E7 LXCXE and the E2F-TD helix motifs are underlined and in bold.
b Values were taken from Ref. 56.
c Measurements were performed at 0.2 m NaCl.