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. 2013 May 15;288(26):18987–18999. doi: 10.1074/jbc.M113.472605

TABLE 2.

Oligonucleotides used in this study

Name Sequence Construction of
CheR2-f 5′-AGAGGCGGCACATATGTCTACGGGTAATTTGG-3′ pET28b-CheR2
CheR2-r 5′-CATCTCTCGATACCGGCCTGGATCCTTACTTG-3′ pET28b-CheR2
CheR3-f 5′-CAACTGGAGCGCATATGACTAGCGAACGCA-3′ pET28b-CheR3
CheR3-f 5′-ACTGCGCGTAGGATCCTCATGATTTACGGA-3′ pET28b-CheR3
mcpT-f 5′-CACATTGAATTCTTAGATAATGAGGTGACAC-3′ pET200/D-TOPO-mcpT
mcpT-r 5′-TTCAGAGGATCCCTAAAGACGGAACATG-3′ pET200/D-TOPO-mcpT
mcpS-f 5′-AGAGCGCATATGAACAGCTGGTTCGCCAACATC-3′ pET28b-mcpS
mcpS-r 5′-TGCGGATCCTCAGACGCGGAACTGGCTGACCAG-3′ pET28b-mcpS
PP3760F 5′-AAGAATTC TTGACTAGCGAACGCAACAC-3′ pChesi-3760
PP3760R 5′-AAGGATCC AGATGATGGTGCGTTCAAGC-3′ pChesi-3760
PP4392F 5′-AAGAATTCGTGTCTACGGGTAATTTGGATTTC-3′ pChesi-4392
PP4390R 5′-AAGGATCC GGCCGAGGTTGCTGCGCTCGAA-3′ pChesi-4392