FIGURE 7.
Production and activity testing of toxin BeM9. A, purification of recombinant toxin after Trx-BeM9 fusion cleavage with CNBr by HPLC. The fraction containing BeM9 is marked by an asterisk. B, representative whole-cell current traces recorded from oocytes expressing cloned Nav isoforms in control and after toxin application. The dotted line indicates the zero current level. Asterisks mark traces after application of 1 μm toxin. Shown are representative traces of at least three independent experiments.