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. 2013 May 15;288(26):19040–19049. doi: 10.1074/jbc.M113.466102

FIGURE 6.

FIGURE 6.

Adenosine signaling counteracts the pro-fibrotic effects of ATP. Adenosine (ado) decreased POM-1-stimulated α-SMA mRNA (A) and protein (B) expression in a concentration-dependent manner. C, incubation with ATP (10 μm) and adenosine (ado, 30 μm) for 4 h increased CREB phosphorylation, an effect that was blunted with the PKA inhibitors, H89 (10 μm) and (Rp)-cAMPS (50 μm). D, inhibition of A2B adenosine receptors with PSB 603 (300 nm) blocked ATP-stimulated CREB phosphorylation; A2A receptor inhibition (300 nm SCH 442416) had no significant effect. *, p < 0.05; **, p < 0.01 versus untreated controls (cont); +, p < 0.05; ++, p < 0.01; +++, p < 0.001 between groups indicated or as compared with control CFs not treated with inhibitors (C). Data are presented as mean ± S.E. of at least three independent experiments. WB, Western blot; veh, vehicle.