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. 2013 May 15;288(26):19040–19049. doi: 10.1074/jbc.M113.466102

FIGURE 7.

FIGURE 7.

Extracellular adenosine deamination and A2B receptor inhibition enhances pro-fibrotic ATP signaling. Incubation with adenosine deaminase (ADA) increased collagen synthesis by 38% (after 24 h) (A) and the expression of α-SMA and PAI-1 protein 2- and 3-fold, respectively (after 4 h) (B). Incubation of CFs with ADA enhanced the stimulation produced by 10 μm ATP in the expression of α-SMA (42%) and PAI-1 (182%). C, incubation with 300 nm PSB 603 (PSB) enhanced the stimulation by ATP (10 μm, 4 h treatment) of α-SMA and PAI-1 gene expression by 62 and 69%, respectively. D, incubation for 24 h with PSB produced a 33% increase in ATP-promoted enhancement in α-SMA protein levels. PSB did not enhance the UTP-promoted increases in α-SMA or PAI-1 expression. E, effects of 30 μm POM-1 were not enhanced by pretreatment with PSB. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus untreated controls (cont); +, p < 0.05; ++, p < 0.01; +++, p < 0.001 between groups indicated. Data are presented as mean ± S.E. of three independent experiments. WB, Western blot.