FIGURE 5.

MIMOOX and Tat variants recognition by anti-MIMOOX serum dilution. ELISA (n = 2) was performed with four dilutions of the anti-MIMOOX rat serum with coated MIMOOX (black squares), Tat OYI (white squares), Tat UG11RP (white triangles), Tat 96BW (white circles), Tat Eli (black triangles), and Tat CM240 (black circles). ELISA was performed also with a negative control (white star) made of a pool of three peptides corresponding respectively to regions 1, 3, and 6 of Tat OYI. The anti-MIMOOX serum recognizes the five Tat variants and MIMOOX. Tat OYI was recognized as well as MIMOOX. Serum dilution shows that Tat Eli is better recognized compared with the other Tat variants. A similar result was observed with a previously published Tat OYI immunization (14).