TABLE 4.
Steady-state kinetic parameters for PenA and PenI with β-lactamase inhibitors
The Km, app value was determined using a direct competition assay under steady-state conditions. kcat/kinact represents the ratio of inhibitor to enzyme (I/E) necessary to inhibit the hydrolysis of NCF by greater than 99%. Each experiment was completed in triplicate, and the error values represent the mean ± S.E.
| Km, app | kcat/kinact | |
|---|---|---|
| μm | ||
| CLAV | ||
| PenA | 3.1 ± 0.3 | 2000 |
| PenI | 2.3 ± 0.2 | 5 |
| SUL | ||
| PenA | 4.1 ± 0.4 | 1500 |
| PenI | 2.4 ± 0.2 | 5 |
| TAZO | ||
| PenA | 0.5 ± 0.1 | 50 |
| PenI | 1.9 ± 0.2 | 1 |