FIGURE 2.

RhoA methylation is dynamic. A, impact of thrombin stimulation on RhoA methylation. MDA-MB-231 cells were starved of serum overnight and then treated with vehicle (Starve) or incubated with thrombin (Thromb.) for 30 min prior to harvest and processing for two-dimensional gel analysis as described under “Experimental Procedures.” B, quantitative representation of unmethylated RhoA levels from A, expressed as a fraction of total RhoA. Data represent the mean ± S.E. from pooled results of two independent experiments. U, unmethylated RhoA; M, methylated RhoA.