TABLE 1.

PCR primers used in cloning of SPR and site-directed mutagenesis

Primer	Sequence
hSPR-F	CCGCGCGGCAGCCATATGGAGGGCGGGCTG
hSPR-R	CGAATTCGGATCCTTATCATTTGTCATAGAAGTC
mSPR-F	CCGCGCGGCAGCCATATGGAGGCAGGCGG
mSPR-R	CGAATTCGGATCCTTATCAGTCATAGAAGTCCAC
G14S-F	GCTTGCTGACCAGCGCCTCCCGCGGC
G14S-R	GCCGCGGGAGGCGCTGGTCAGCAAGC
G18D-F	GGCCTCCCGCGACTTCGGCCGGACG
G18D-R	CGTCCGGCCGAAGTCGCGGGAGGCC
R42G-F	GTCCTTAGCGCCGGCAACGACGAGGC
R42G-R	GCCTCGTCGTTGCCGGCGCTAAGGAC
N99A-F	GCTTATCAACGCCGCGGGCTCTCTTG
N99A-R	CAAGAGAGCCCGCGGCGTTGATAAGC
S157A-F	GTTAACATCTCGGCCCTCTGTGCCCTGC
S157A-R	GCAGGGCACAGAGGGCCGAGATGTTAAC
K174L-F	GTACTGTGCAGGACTGGCTGCTCGTG
K174L-R	CACGAG CAGCCAGTCC TGCACAGTAC
M205G-F	GGACACAGACGGGCAGCAGTTGGC
M205G-R	GCCAACTGCTGCCCGTCTGTGTCC
D257H-R	CGAATTCGGATCCTTATCATTTGTCATAGAAGTGCACGTGGGCTCC
DEL257-R	CGAATTCGGATCCTTATCACACGTGGGCTCC
Y259A-R	CGAATTCGGATCCTTATCATTTGTCAGCGAAGTCCACGTGGGCTCC