FIGURE 1.

Cpc2 is important for induction of amino acid biosynthesis genes. Gene expression levels of nine GAAC genes and cdk9⁺ (control) were analyzed by quantitative RT-PCR from wild-type (YT3033), cpc2Δ (YT2307), and gcn2Δ (YT3360) cells treated with 10 mm 3-aminotriazole (3AT) for 0 min (white bar), 15 min (gray bar), and 60 min (black bar). All data are normalized to the expression level of 18 S rRNA and shown as the relative fold to those at time 0 in wild-type cells with S.E. (n = 3). GAAC genes are: his4⁺, imidazoleglycerol-phosphate synthase (predicted); SPAC56E4.03, aromatic aminotransferase (predicted); SPCC364.07, d-3 phosphoglycerate dehydrogenase (predicted); SPAC10F6.13c, aspartate aminotransferase (predicted); arg3⁺, ornithine carbamoyltransferase; dld1⁺, dihydrolipoamide dehydrogenase; lys3⁺, saccharopine dehydrogenase; leu3⁺, 2-isopropylmalate synthase; SPBC19F5.04, aspartate kinase (predicted).