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. 2013 May 13;288(26):19260–19268. doi: 10.1074/jbc.M112.445270

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Cpc2 is required for starvation-induced Gcn2 activation. A, immunoblots of phosphorylated eIF2α and Cdc2 (control) from Flag:gcn2 (YT3372), Flag:gcn2_TA (YT3657), and Flag:gcn2_KR (YT3656) cells treated with 10 mm 3AT. B, wild-type (JK317), gcn2Δ (YT3360), Flag:gcn2 (YT3372), Flag:gcn2_TA (YT3657), and Flag:gcn2_KR (YT3656) cells were 10-fold serially diluted and spotted on EMM plates ± 10 mm 3AT. C, immunoblots of anti-FLAG-Gcn2 immunoprecipitates from Flag:gcn2 (YT3372), Flag:gcn2 cpc2Δ (YT3598), and Flag:gcn2_TA (YT3657) cells treated with 10 mm 3AT, probed with anti-phospho-Gcn2 and anti-FLAG (control) antibodies.